Phd thesis on cellulase

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Sustainable energy sources, such as biofuels, offer increasingly important alternatives to fossil fuels that contribute less to global climate change. The energy contained within cellulosic biofuels derives from sunlight energy stored in the form of carbon-carbon bonds comprising sugars such as glucose. Second-generation biofuels are produced from lignocellulosic biomass feedstocks, including agricultural waste products and non-food crops like Miscanthus, that contain lignin and the polysaccharides hemicellulose and cellulose. Cellulose is the most abundant biological material on Earth; it is a polymer of glucose and a structural component of plant cell walls. Accessing the sugar is challenging, as the crystalline structure of cellulose resists degradation; biochemical and thermochemical means can be used to depolymerize cellulose. Cellulase enzymes catalyze the biochemical depolymerization of cellulose into glucose. Glucose can be used as a carbon source for growth of a biofuel-producing microorganism.
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Protoplast fusion and genetic analysis in Cephalosporium acremonium. Although high protoplast yields could be obtained with the laboratory preparation from Cytophaga it would be much more convenient to use a commercial product. Several enzymes, i. It seemed possible that the latter products could contain side activities capable of digesting fungal cell walls. The effectiveness of these enzymes in releasing protoplasts from C.
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